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MBC in Press, published online ahead of print November 19, 2008
Mol. Biol. Cell 10.1091/mbc.E08-07-0746

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Submitted on July 21, 2008
Revised on October 28, 2008
Accepted on November 7, 2008

Dynein-2 Affects the Regulation of Ciliary Length but Is Not Required for Ciliogenesis in Tetrahymena thermophila

Vidyalakshmi Rajagopalan,* Aswati Subramanian,{dagger} David E. Wilkes,* David G. Pennock,{dagger} and David J. Asai*{ddagger}

*Department of Biology, Harvey Mudd College, Claremont, CA, 91711; {dagger}Department of Zoology, Miami University, Oxford, OH, 45056; {ddagger}Howard Hughes Medical Institute, Chevy Chase, MD, 20815

Monitoring Editor: Paul Forscher

Eukaryotic cilia and flagella are assembled and maintained by the bidirectional intraflagellar transport (IFT). Studies in alga, nematode, and mouse have shown that the heavy chain (Dyh2) and the light IC (D2LIC) of the cytoplasmic dynein-2 complex are essential for retrograde intraflagellar transport. In these organisms, disruption of either dynein-2 component results in short cilia/flagella with bulbous tips in which excess IFT particles have accumulated. In Tetrahymena, the expression of the DYH2 and D2LIC genes increases during reciliation, consistent with their roles in IFT. However, the targeted elimination of either DYH2 or D2LIC gene resulted in only a mild phenotype. Both knockout cell lines assembled motile cilia but the cilia were of more variable lengths and less numerous than wild-type controls. Electron microscopy revealed normally shaped cilia with no swelling and no obvious accumulations of material in the distal ciliary tip. These results demonstrate that dynein-2 contributes to the regulation of ciliary length but is not required for ciliogenesis in Tetrahymena. Loading domain annotation.

Address correspondence to: David J. Asai (david_asai{at}hmc.edu)






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