Molecular Biology of the Cell

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print November 19, 2008
Mol. Biol. Cell 10.1091/mbc.E08-06-0628

This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Materials
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Liu, H.-L.
Right arrow Articles by Osmani, S. A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, H.-L.
Right arrow Articles by Osmani, S. A.

Submitted on June 20, 2008
Revised on October 20, 2008
Accepted on November 10, 2008

The Three Fungal Transmembrane Nuclear Pore Complex Proteins of Aspergillus nidulans Are Dispensable in the Presence of an Intact An-Nup84–120 Complex

Hui-Lin Liu, Colin P.C. De Souza, Aysha H. Osmani, and Stephen A. Osmani

Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210

Monitoring Editor: Reid Gilmore

In Aspergillus nidulans nuclear pore complexes (NPCs) undergo partial mitotic disassembly such that 12 NPC proteins (Nups) form a core structure anchored across the nuclear envelope (NE). To investigate how the NPC core is maintained, we affinity purified the major core An-Nup84–120 complex and identified two new fungal Nups, An-Nup37 and An-ELYS, previously thought to be vertebrate specific. During mitosis the An-Nup84–120 complex locates to the NE and spindle pole bodies but, unlike vertebrate cells, does not concentrate at kinetochores. We find that mutants lacking individual An-Nup84–120 components are sensitive to the membrane destabilizer benzyl alcohol (BA) and high temperature. Although such mutants display no defects in mitotic spindle formation, they undergo mitotic specific disassembly of the NPC core and transient aggregation of the mitotic NE suggesting the An-Nup84–120 complex might function with membrane. Supporting this, we show cells devoid of all known fungal transmembrane Nups (An-Ndc1, An-Pom152 and An-Pom34) are viable but that An-ndc1 deletion combined with deletion of individual An-Nup84–120 components is either lethal or causes sensitivity to treatments expected to destabilize membrane. Therefore the An-Nup84–120 complex performs roles, perhaps at the NPC membrane as previously proposed, that become essential without the An-Ndc1 transmembrane Nup.

Address correspondence to: Stephen A. Osmani (osmani.2{at}osu.edu)






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2008 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.