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MBC in Press, published online ahead of print November 12, 2008
Mol. Biol. Cell 10.1091/mbc.E08-04-0360

A more recent version of this article appeared on January 1, 2009
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Submitted on April 8, 2008
Revised on October 27, 2008
Accepted on October 30, 2008

A Dual Role for Actin and Microtubule Cytoskeleton in the Transport of Golgi Units from the Nurse Cells to the Oocyte Across Ring Canals

Emmanuelle Nicolas,* Nicolas Chenouard,{dagger} Jean-Christophe Olivo-Marin,{dagger} and Antoine Guichet*

*Morphogenesis and Polarity Unit, Institut Jacques Monod, 75005 Paris France; {dagger}Quantitative Image Analysis Unit, Institut Pasteur, 75724 Paris, France

Monitoring Editor: Vivek Malhotra

Axis specification during Drosophila embryonic development requires transfer of maternal components during oogenesis from nurse cells (NC) into the oocyte through cytoplasmic bridges. We found that the asymmetrical distribution of Golgi, between nurse cells and the oocyte, is sustained by an active transport process. We have caracterized actin basket structures that asymmetrically cap the NC side of Ring canals (RC) connecting the oocyte. Our results suggest that these actin baskets structurally support transport mechanisms of RC transit. In addition, our tracking analysis indicates that Golgi units are actively transported to the oocyte rather than diffusing. We observed that RC transit is microtubules-based and mediated at least by Dynein. Finally, we show that actin networks may be involved in RC cross-ing through a Myosin II step process, as well as in dispatching Golgi units inside the oocyte subcompartments.

Address correspondence to: Antoine Guichet (guichet{at}ijm.jussieu.fr)






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