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Vol. 11, Issue 7, 2221-2233, July 2000
Department of Biology, Graduate School of Science, Osaka
University, Osaka 560-0043, Japan
The MRE11, RAD50, and
XRS2 genes of Saccharomyces cerevisiae
are involved in the repair of DNA double-strand breaks (DSBs) produced
by ionizing radiation and by radiomimetic chemicals such as methyl
methanesulfonate (MMS). In these mutants, single-strand DNA degradation
in a 5' to 3' direction from DSB ends is reduced. Multiple copies of
the EXO1 gene, encoding a 5' to 3' double-strand DNA
exonuclease, were found to suppress the high MMS sensitivity of these
mutants. The exo1 single mutant shows weak MMS
sensitivity. When an exo1 mutation is combined with an
mre11 mutation, both repair of MMS-induced damage and
processing of DSBs are more severely reduced than in either single
mutant, suggesting that Exo1 and Mre11 function independently in DSB
processing. During meiosis, transcription of the EXO1
gene is highly induced. In meiotic cells, the exo1
mutation reduces the processing of DSBs and the frequency of crossing
over, but not the frequency of gene conversion. These results suggest
that Exo1 functions in the processing of DSB ends and in meiotic
crossing over.
Iwate
College of Nursing, 14-1 Sengakubo, Ohgama, Takizawa, Iwate 020-0151, Japan.
Corresponding author. E-mail address:
hogawa{at}iwate-nurse.ac.jp.
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